Evaluating the radioprotective effect of single dose and daily oral consumption of green tea, grape seed, and coffee bean extracts against gamma irradiation. (August 2021)

• Record Type: Journal Article
• Authors: Ansari, Leyla; Banaei, Amin; Dastranj, Leila; Majdaeen, Mehrsa; Vafapour, Hassan; Zamani, Hamed; Ataei, Gholamreza; Abedi-Firouzjah, Razzagh
• Is Part Of: Applied radiation and isotopes. Volume 174(2021)
• Publication Date: 2021-08
• Subjects: Green tea -- Grape seed -- Coffee bean extract -- Radiation protection -- Micronucleus assessment -- Comet assay; Radiology -- Periodicals; Radiation -- Industrial applications -- Periodicals; Nuclear chemistry -- Periodicals; Internet resource; Periodical; 660.298
• DOI: 10.1016/j.apradiso.2021.109781
• Languages: English
• ISSNs: 0969-8043

Abstract

Abstract: Introduction: The aim of this study was to investigate and compare the radio-protective effect of green tea, grape seed, and coffee bean extracts in different oral consumption methods in mice. Materials and methods: In this experimental-quantitative study 150 mice in 15 equally sized groups were used. For each extract, two groups received 200 mg/kg of herbal extracts' combination for 7 and 30 consecutive days before irradiation, and one group received 800 mg/kg of the extract 2 h before irradiation (3 Gy gamma-rays of Co-60). The similar groups were classified to receive a combination of the plant extracts (green tea, grape seed, and coffee bean). Irradiation without consuming plant extract (irradiated group), and a control group were also devised. Alkaline comet and micronucleus assays were used to investigate the radioprotective effect on mice blood and bone marrow cells, respectively. Results: Consumption of all plant extracts significantly decreased the radiation damage to blood and bone marrow cells, compared to the irradiated group (p < 0.01), with grape seed extract showing higher protective effect. Continuous daily oral consumption (one week/month) showed a significant higher radioprotective effect compared to single consumption (p < 0.05). Continuous consumption of the combination of the extracts showed a higher radio-protection in comparison to each of the plant extracts (p < 0.03). Conclusions: The radioprotective effect of continuous consumption (for one week/month) of the plant extracts was greater than single dose. In continuous consumption protocols, we found the synergetic property and higher radioprotective effect of the plant extract combination compared to each one. Highlights: Investigating the radio-protective effect of green tea, grape seed, and coffee bean extracts in oral consumption methods. Alkaline comet and micronucleus assays were used. Consumption of all plant extracts significantly decreased the radiation damage to blood and bone marrow cells. Continuous daily oral consumption showed a significant higher radioprotective effect compared to single consumption.