A simple, rapid and sensitive method for the simultaneous determination of eighteen environmental phenols in human urine. (September 2021)

• Record Type: Journal Article
• Authors: Ao, Junjie; Zhang, Qianlong; Tang, Weifeng; Yuan, Tao; Zhang, Jun
• Is Part Of: Chemosphere. Volume 278(2021)
• Publication Date: 2021-09
• Subjects: Paraben -- Bisphenol -- Benzophenone -- Triclosan -- Human urine -- Biomonitoring; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2021.130494
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Environmental phenols are a typical group of endocrine disrupting compounds (EDCs) and have caused growing concerns upon the potential adverse effects on humans. Urinary concentrations of phenols can be used as valid biomarkers for the assessment of human exposure. A method was developed for the simultaneous determination of eighteen environmental phenols (six parabens, seven bisphenols, four benzophenones and triclosan) in human urine using liquid-liquid extraction (LLE) coupled to high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The optimized LLE was time saving and required low volumes of organic solvents. A volume of only 0.2 mL urine sample was extracted and analyzed. The method yielded good linearity (0.9925–0.9994) and satisfactory limit of detection (LOD) (≤0.08 ng mL −1 ). The relative recoveries ranged between 76.7% and 116% at three spiked levels, with intra- and inter-day precision less than 8.04% and 13.5%, respectively. Our method has been proved to be simple, rapid and sensitive by a comprehensive comparison between methods. This proposed method provides a large-scale biomonitoring tool for exposure assessment of human population to environmental phenols. Graphical abstract: Image 1 Highlights: A method for the simultaneous determination of 18 phenols in urine was developed. A volume of only 0.2 mL urine sample was required. The optimized liquid-liquid extraction was time and organic solvents saving. Comprehensive comparison proved that the method is simple, rapid and sensitive.