Disentangling the abiotic and biotic components of AMF suppressive soils. (August 2021)

• Record Type: Journal Article
• Authors: Cruz-Paredes, Carla; Diera, Tomas; Davey, Marie; Rieckmann, Maria Monrad; Christensen, Peter; Dela Cruz, Majbrit; Laursen, Kristian Holst; Joner, Erik J.; Christensen, Jan H.; Nybroe, Ole; Jakobsen, Iver
• Is Part Of: Soil biology and biochemistry. Volume 159(2021)
• Publication Date: 2021-08
• Subjects: Aluminum -- Arbuscular mycorrhizal fungi -- Fusarium -- pH -- Soil suppression -- Volatile organic compounds; Soil biochemistry -- Periodicals; Soil biology -- Periodicals; Sols -- Biochimie -- Périodiques; Sols -- Biologie -- Périodiques; Sols -- Microbiologie -- Périodiques; Bodembiologie; Biochemie; 631.46
• DOI: 10.1016/j.soilbio.2021.108305
• Languages: English
• ISSNs: 0038-0717

Abstract

Abstract: Arbuscular mycorrhizal fungi (AMF) are important in plant nutrient uptake, but their function is prone to environmental constraints including soil factors that may suppress AMF transfer of phosphorus (P) from the soil to the plant. The objective of this study was to disentangle the biotic and abiotic components of AMF-suppressive soils. Suppression was measured in terms of AMF-mediated plant uptake of 33 P mixed into a patch of soil and treatments included soil sterilization, soil mixing, pH manipulation and inoculation with isolated soil fungi. The degree of suppression was compared to volatile organic compound (VOC) production by isolated fungi and to multi-element analysis of soils. For a selected suppressive soil, sterilization and soil mixing experiments confirmed a biotic component of suppression. A Fusarium isolate from that soil suppressed the AMF activity and produced greater amounts than other fungal isolates of the antimicrobial VOC trichodiene (a trichothecene toxin precursor), beta-chamigrene, alpha-cuprenene and p-xylene. These metabolites deserve further attention when unravelling the chemical background behind the suppression of AMF activity by soil microorganisms. For the abiotic component of suppression, soil liming and acidification experiments confirmed that suppression was strongest at low pH. The pH effect might be associated with changed availability of specific suppressive elements. Indeed 33 P uptake from the soil patches correlated negatively to Al levels and Al toxicity seems to play a major role in the AMF suppressiveness at pH below 5.0–5.2. However, the documentation of a biotic component of suppression for both low and high pH soils leads to the conclusion that biotic and abiotic components of suppression may act in parallel in some soils. The current insight into the components of soil suppressiveness of the AMF activity aids to develop management practices that allow for optimization of AMF functionality. Highlights: In some soils, suppression of AMF activity had a biotic component. A Fusarium isolate from a suppressive soil inhibited the AMF activity. This isolate produced greater amounts of antimicrobial VOCs than other isolates. AMF suppression increased at low pH across several soils. For these soils, Al toxicity played a major role in suppressiveness at pH ≤ 5.0