Extracellular polymeric substances excreted by anammox sludge act as a barrier for As(III) invasion: Binding property and interaction mechanism. (September 2021)

• Record Type: Journal Article
• Authors: Ma, Wen-Jie; Zhang, Jiang-Tao; Wang, Ye; Li, Gui-Feng; Wu, Xin-Xin; Yao, Yu-Xi; Cheng, Ya-Fei; Huang, Bao-Cheng; Jin, Ren-Cun
• Is Part Of: Chemosphere. Volume 278(2021)
• Publication Date: 2021-09
• Subjects: Anammox -- Arsenic -- Extracellular polymeric substance -- Interaction; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2021.130414
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: The arsenic in livestock wastewater would induce adverse impact on the biological treatment technology such as anaerobic ammonium oxidation (anammox) process. Extracellular polymeric substances (EPS) play an important role in resisting such toxicity. Unfortunately, the role of EPS in protecting anammox from As(III) and the mechanisms underlying the protection still remains unclear. This work comprehensively evaluated the acute toxicity of arsenic on anammox sludge and investigated the binding property and interaction mechanism. The results revealed that the half maximal inhibitory concentration (IC50 ) of As(III) on anammox sludge was estimated to be 408 mg L −1, which decreased to 41.97 mg L −1 when EPS was exfoliated. Complexation and hydrophobic interactions were the leading forces in preventing arsenic invasion. Protein was the main component that complexes with As(III), and O–H, –NH, –CO were binding sites. The response sequence of organic component in EPS to As(III) was ordered as hydrocarbons-proteins-polysaccharides-aliphatic amines. Graphical abstract: Image 1 Highlights: The IC50 of As(III) was estimated as 408 mg L −1 in anammox process. EPS was able to construct buffer zones to prevent As(III) invasion. Protein is the main component within EPS to complex with As(III). Response sequence of functional groups to As(III) was clarified via spectra analysis.