Effect of miR‐630 expression on esophageal cancer cell invasion and migration. Issue 6 (21st May 2021)

• Record Type: Journal Article
• Authors: Liu, Xi; Wu, Wei; Zhang, Shixin; Tan, Wenfeng; Qiu, Yang; Liao, Kelong; Yang, Kang
• Is Part Of: Journal of clinical laboratory analysis. Volume 35:Issue 6(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-05-21
• Subjects: diagnostic value -- esophageal cancer -- invasion -- migration -- miR‐630; Diagnosis, Laboratory -- Periodicals; Medical laboratory technology -- Periodicals; 616
• DOI: 10.1002/jcla.23815
• Languages: English
• ISSNs: 0887-8013

Abstract

Abstract: Background: Esophageal cancer (EC) is a common malignancy of the digestive tract, with high incidence. The objective of this study was to investigate the effect of miR‐630 expression on esophageal cancer (EC) cell invasion and migration. Methods: The study group comprised 58 EC patients admitted to our hospital from April 2014 to 2016, and the control group comprised 60 healthy people visiting the hospital during the same period. miR‐630 levels in the peripheral blood of the two groups were compared, and the diagnostic value of miR‐630 for EC was analyzed. EC cell lines were used to evaluate the influence of miR‐630 expression on EC cell invasion and migration. Results: miR‐630 expression was low in EC ( p < 0.050). A receiver operating characteristic curve analysis showed that miR‐630 expression had a good diagnostic value for EC ( p < 0.050) and was associated with disease course, pathological stage, differentiation degree, tumor metastasis, and patient prognosis and survival ( p < 0.05). The ROC curve analysis showed that when cutoff value was 5.38, the diagnostic sensitivity and specificity of miR‐630 for EC were 73.33% and 76.67%, respectively; area under the ROC curve was 0.778 (95%CI 0.695–0.861). Transfection of miR‐630 into EC cells indicated that miR‐630 overexpression can reduce EC cell invasion and migration ( p < 0.05). miR‐630 expression is low in EC and has good diagnostic value for EC. Conclusion: miR‐630 overexpression can reduce EC cell invasion and migration, showing a possible key role of miR‐630 in EC diagnosis and treatment in the future. Abstract : Biological effect of miR‐630 expression on EC cells. (A) miR‐630 expression in different EC cell lines; * indicates comparison with KYSE‐150 cell line, p < 0.05; # indicates comparison with the KYSE‐450 cell line, p < 0.05; @ indicates comparison with the ECA109 cell line, p < 0.05. (B) Detection of cell migration ability. * indicates comparison with the miR‐630‐mimics group, p < 0.05; # indicates comparison with the miR‐NC group, p < 0.05. (C) Detection of cell invasion capability. * indicates comparison with the miR‐630‐mimics group, p < 0.05; # indicates comparison with the miR‐630‐inhibit group, p < 0.05.