A Ratio Fluorescent Probe for the Detection of Bisulfite and Its Application in Living Cells. Issue 18 (12th May 2021)

• Record Type: Journal Article
• Authors: Zhao, Xuejun; Shen, Qingguo; Sun, Yan; Han, Xueshun; Guan, Yongxia
• Is Part Of: ChemistrySelect. Volume 6:Issue 18(2021)
• Page Start: 4418
• Page End: 4423
• Publication Date: 2021-05-12
• Subjects: Keyword: Biological -- Bisulfite/sulfite -- Fluorescent probe -- Ratiometric; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/slct.202100843
• Languages: English
• ISSNs: 2365-6549

Abstract

Abstract: Sulfur dioxide derivatives play excellently vital roles in numerous essential physiology and pathology processes of living systems. The abnormal levels of bisulfite/sulfite (HSO3 − /SO3 2− ) may cause the occurrence of a series of diseases, including acute symptoms of respiratory disease, brain damage, neurological disorders, cardiovascular disease, and even cancer. Therefore, it is necessary to develop simple and effective methods to monitor the fluctuation of HSO3 − /SO3 2− . Herein, a novel ratiometric fluorescent probe ((E)‐3‐(2‐(4‐formylstyryl)‐3, 3‐dimethyl‐3H‐benzo[g]indol‐1‐ium‐1‐yl)propane‐1‐sulfonate, Cy‐DBPS ) is devised to trace HSO3 − /SO3 2− in living cells. In the presence of HSO3 − /SO3 2−, the large conjugated system is destroyed by triggering the Michael addition reaction, which further induce the probe to emit a ratio of fluorescence signal. Furthermore, the probe can quickly respond to HSO3 − /SO3 2− within 120 s and exhibits low detection limit, which provides the possibility of sensitive and real‐time detection of HSO3 − /SO3 2− in living cells. Based on the unique advantages of high selectivity, negligible cytotoxicity and great biocompatibility, Cy‐DBPS can effectively detect exogenous and endogenous HSO3 − /SO3 2− in HepG2 and HeLa cells. The outstanding properties of the probe Cy‐DBPS provided a great potential for the further valuable application in living cells and even deeper biological applications for HSO3 − /SO3 2− detection. Abstract : We developed a novel ratiometric fluorescent probe, (E)‐3‐(2‐(4‐formylstyryl)‐3, 3‐dimethyl‐3H‐benzo[g]indol‐1‐ium‐1‐yl)propane‐1‐sulfonate (Cy‐DBPS), to visualize and track the content of HSO3 − in living cells. In the presence of HSO3 − /SO3 2−, causing the large conjugated system is destroyed by triggering the Michael addition reaction, further induces the probe to emit a ratio of fluorescence signal. Moreover, the proposed probe can quickly respond to HSO3 − and display high selectivity, negligible cytotoxicity and great biocompatibility.