A Novel GABABR1a Receptor Electrochemical Biosensor Based on Gold Nanoparticles Chitosan‐horseradish Peroxidase. Issue 6 (30th March 2021)

• Record Type: Journal Article
• Authors: Zhao, Xiaotong; Wei, Lihui; Pang, Guangchang; Xie, Junbo
• Is Part Of: Electroanalysis. Volume 33:Issue 6(2021)
• Page Start: 1606
• Page End: 1614
• Publication Date: 2021-03-30
• Subjects: Nano biosensor -- GABABR1a -- Jujuboside A -- Baclofen; GPCRs; Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.202060594
• Languages: English
• ISSNs: 1040-0397

Abstract

Abstract: A novel gamma aminobutyric acid type B receptor subunit 1a (GABAB R1a) biosensor modified with double‐layer gold has been developed for the first time. In this work, the synthesized plasmid was transfected into HEK‐293T cells by liposome transfection, and the target protein GABAB R1a was obtained. The horseradish peroxidase was used as a signal amplification system to produce a double‐layer gold modified GABAB R1a receptor biosensor. The ligand gamma aminobutyric acid (GABA), jujuboside A and baclofen were detected by the time‐current method with the biosensor. The results showed that the action regularity of GABA, jujuboside A, and baclofen on GABAB R1a receptor was fitted with hyperbolic fitting (R 2 was 0.9740, 0.9770 and 0.9770, respectively). The double reciprocal method was used to figure out the affinity constant (Ka ) of GABA, jujuboside A, and baclofen with the receptor, i. e. 2.1016×10 −15 mol/L, 1.7601×10 −14 mol/L, and 1.633×10 −14 mol/L, respectively, indicating that these measured compounds exhibited different action strength on the receptor. Based on the above, this study will provide a basis for drug screening and will also provide a new idea and method for investigating quantitatively the interaction between receptors and ligands in the future. Abstract :