Biopolymer/Glycopolypeptide‐Blended Scaffolds: Synthesis, Characterization and Cellular Interactions. Issue 24 (5th December 2019)

• Record Type: Journal Article
• Authors: Dhaware, Vinita; Díaz Díaz, David; Sen Gupta, Sayam
• Is Part Of: Chemistry, an Asian journal. Volume 14:Issue 24(2019)
• Page Start: 4837
• Page End: 4846
• Publication Date: 2019-12-05
• Subjects: Adhesion -- Aggregates -- Cellular infiltration -- Spheroids; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/asia.201901227
• Languages: English
• ISSNs: 1861-4728

Abstract

Abstract: Three‐dimensional (3D) scaffolds formed from natural biopolymers gelatin and chitosan that are chemically modified by galactose have shown improved hepatocyte adhesion, spheroid geometry and functions of the hepatocytes. Galactose specifically binds to the hepatocytes via the asialoglycoprotein receptor (ASGPR) and an increase in galactose density further improves the hepatocyte proliferation and functions. In this work, we aimed to increase the galactose density within the biopolymeric scaffold by physically blending the biopolymers chitosan and gelatin with an amphiphlic β‐galactose polypeptide (PPO‐GP). PPO‐GP, is a di‐block copolymer with PPO and β‐galactose polypeptide, exhibits lower critical solution temperature and is entrapped within the scaffold through hydrophobic interactions. The uniform distribution of PPO‐GP within the scaffold was confirmed by fluorescence microscopy. SEM and mechanical testing of the hybrid scaffolds indicated pore size, inter connectivity and compression modulus similar to the scaffolds made from 100 % biopolymer. The presence of the PPO‐GP on the surface of the scaffold was tested monitoring the interaction of an analogous mannose containing PPO‐GP scaffold and the mannose binding lectin Con‐A. In vitro cell culture experiments with HepG2 cells were performed on GLN‐GP and CTS‐GP and their cellular response was compared with GLN and CTS scaffolds for a period of seven days. Within three days of culture the Hep G2 cells formed multicellular spheroids on GLN‐GP and CTS‐GP more efficiently than on the GLN and CTS scaffolds. The multicellular spheroids were also found to infiltrate more in GLN‐GP and CTS‐GP scaffolds and able to maintain their round morphology as observed by live/dead and SEM imaging. Abstract : 3D porous scaffolds were formed by physically blending amphiphilic poly(propylene oxide)‐β‐galactose polypeptide (PPO‐GP) to increase the galactose densities within the scaffolds formed, using natural gelatin and chitosan biopolymers. The hybrid scaffolds were found to have interconnected heterogeneous pores and a compressive modulus comparable to the liver tissue.