Enhancing glutaric acid production in Escherichia coli by uptake of malonic acid. (1st March 2020)

• Record Type: Journal Article
• Authors: Sui, Xue; Zhao, Mei; Liu, Yingli; Wang, Jing; Li, Guohui; Zhang, Xiaojuan; Deng, Yu
• Is Part Of: Journal of industrial microbiology & biotechnology. Volume 47:Number 3(2020)
• Page Start: 311
• Page End: 318
• Publication Date: 2020-03-01
• Subjects: Glutaric acid -- Escherichia coli -- Clover rhizobia -- Reversed adipate degradation pathway -- Malonic acid -- Malonyl-CoA; Industrial microbiology -- Periodicals; 660.62
• DOI: 10.1007/s10295-020-02268-6
• Languages: English
• ISSNs: 1367-5435

Abstract

Abstract: Glutaric acid is an important organic acid applied widely in different fields. Most previous researches have focused on the production of glutaric acid in various strains using the 5-aminovaleric acid (AMV) or pentenoic acid synthesis pathways. We previously utilized a five-step reversed adipic acid degradation pathway (RADP) in Escherichia coli BL21 (DE3) to construct strain Bgl146. Herein, we found that malonyl-CoA was strictly limited in this strain, and increasing its abundance could improve glutaric acid production. We, therefore, constructed a malonic acid uptake pathway in E. coli using matB (malonic acid synthetase) and matC (malonic acid carrier protein) from Clover rhizobia . The titer of glutaric acid was improved by 2.1-fold and 1.45-fold, respectively, reaching 0.56 g/L and 4.35 g/L in shake flask and batch fermentation following addition of malonic acid. Finally, the highest titer of glutaric acid was 6.3 g/L in fed-batch fermentation at optimized fermentation conditions.