Isolation of a novel platform bacterium for lignin valorization and its application in glucose-free cis, cis-muconate production. (1st August 2019)

• Record Type: Journal Article
• Authors: Shinoda, Eri; Takahashi, Kenji; Abe, Nanase; Kamimura, Naofumi; Sonoki, Tomonori; Masai, Eiji
• Is Part Of: Journal of industrial microbiology & biotechnology. Volume 46:Number 8(2019)
• Page Start: 1071
• Page End: 1080
• Publication Date: 2019-08-01
• Subjects: cis, cis-Muconate -- Lignin valorization -- Pseudomonas; Industrial microbiology -- Periodicals; 660.62
• DOI: 10.1007/s10295-019-02190-6
• Languages: English
• ISSNs: 1367-5435

Abstract

Abstract: Microbial production of cis, cis -muconate (ccMA) from phenolic compounds obtained by chemical depolymerization of lignin is a promising approach to valorize lignin. Because microbial production requires a large amount of carbon and energy source, it is desirable to establish a ccMA-producing strain that utilizes lignin-derived phenols instead of general sources like glucose. We isolated Pseudomonas sp. strain NGC7 that grows well on various phenolic compounds derived from p -hydroxyphenyl, guaiacyl, and syringyl units of lignin. An NGC7 mutant of protocatechuate (PCA) 3, 4-dioxygenase and ccMA cycloisomerase genes (NGC703) lost the ability to grow on vanillate and p -hydroxybenzoate but grew normally on syringate. Introduction of a plasmid carrying genes encoding PCA decarboxylase, flavin prenyltransferase, vanillate O -demethylase, and catechol 1, 2-dioxygenase into NGC703 enabled production of 3.2 g/L ccMA from vanillate with a yield of 75% while growing on syringate. This strain also produced ccMA from birch lignin-derived phenols. All these results indicate the utility of NGC7 in glucose-free ccMA production.