Microbial production of O-methylated flavanones from methylated phenylpropanoic acids in engineered Escherichia coli. (1st December 2019)

• Record Type: Journal Article
• Authors: Cui, Heqing; Song, Myoung Chong; Lee, Ji Young; Yoon, Yeo Joon
• Is Part Of: Journal of industrial microbiology & biotechnology. Volume 46:Number 12(2019)
• Page Start: 1707
• Page End: 1713
• Publication Date: 2019-12-01
• Subjects: Methylated flavanone -- Microbial production -- Homoeriodictyol -- Phenylpropanoic acid -- Escherichia coli; Industrial microbiology -- Periodicals; 660.62
• DOI: 10.1007/s10295-019-02239-6
• Languages: English
• ISSNs: 1367-5435

Abstract

Abstract: Methylated flavonoids possess improved bioactivities compared to their unmethylated counterparts. In this study, for the efficient production of O -methylated flavonoids from simple methylated phenylpropanoic acids, a recombinant Escherichia coli strain expressing 4-coumarate:coenzyme A ligase (4CL) from Oryza sativa and chalcone synthase (CHS) from Hordeum vulgare was constructed; this strain produced significant amount of homoeriodictyol (~ 52 mg/L) as well as a few amount of hesperetin (0.4 mg/L), respectively, from ferulic acid and 4-methylcaffeic acid. This demonstrates, for the first time, that the scarce but valuable methylated flavanones can be successfully produced from methylated phenylpropanoic acids in a microbial host via an artificial biosynthetic pathway consisting of 4CL and CHS that can accept O -methylated precursors.