In vivo biosensors: mechanisms, development, and applications. (1st July 2018)

• Record Type: Journal Article
• Authors: Shi, Shuobo; Ang, Ee Lui; Zhao, Huimin
• Is Part Of: Journal of industrial microbiology & biotechnology. Volume 45:Number 7(2018)
• Page Start: 491
• Page End: 516
• Publication Date: 2018-07-01
• Subjects: Biosensor -- Metabolic engineering -- Synthetic biology -- Metabolite sensing; Industrial microbiology -- Periodicals; 660.62
• DOI: 10.1007/s10295-018-2004-x
• Languages: English
• ISSNs: 1367-5435

Abstract

Abstract: In vivo biosensors can recognize and respond to specific cellular stimuli. In recent years, biosensors have been increasingly used in metabolic engineering and synthetic biology, because they can be implemented in synthetic circuits to control the expression of reporter genes in response to specific cellular stimuli, such as a certain metabolite or a change in pH. There are many types of natural sensing devices, which can be generally divided into two main categories: protein-based and nucleic acid-based. Both can be obtained either by directly mining from natural genetic components or by engineering the existing genetic components for novel specificity or improved characteristics. A wide range of new technologies have enabled rapid engineering and discovery of new biosensors, which are paving the way for a new era of biotechnological progress. Here, we review recent advances in the design, optimization, and applications of in vivo biosensors in the field of metabolic engineering and synthetic biology.