High-level recombinant production of squalene using selected Saccharomyces cerevisiae strains. (1st April 2018)

• Record Type: Journal Article
• Authors: Han, Jong Yun; Seo, Sung Hwa; Song, Jae Myeong; Lee, Hongweon; Choi, Eui-Sung
• Is Part Of: Journal of industrial microbiology & biotechnology. Volume 45:Number 4(2018)
• Page Start: 239
• Page End: 251
• Publication Date: 2018-04-01
• Subjects: Squalene -- Saccharomyces cerevisiae -- Metabolic engineering -- Farnesyl diphosphate synthase -- HMG-CoA reductase; Industrial microbiology -- Periodicals; 660.62
• DOI: 10.1007/s10295-018-2018-4
• Languages: English
• ISSNs: 1367-5435

Abstract

Abstract: For recombinant production of squalene, which is a triterpenoid compound with increasing industrial applications, in microorganisms generally recognized as safe, we screened Saccharomyces cerevisiae strains to determine their suitability. A strong strain dependence was observed in squalene productivity among Saccharomyces cerevisiae strains upon overexpression of genes important for isoprenoid biosynthesis. In particular, a high level of squalene production (400 ± 45 mg/L) was obtained in shake flasks with the Y2805 strain overexpressing genes encoding a bacterial farnesyl diphosphate synthase ( ispA ) and a truncated form of hydroxyl-3-methylglutaryl-CoA reductase ( tHMG1 ). Partial inhibition of squalene epoxidase by terbinafine further increased squalene production by up to 1.9-fold (756 ± 36 mg/L). Furthermore, squalene production of 2011 ± 75 or 1026 ± 37 mg/L was obtained from 5-L fed-batch fermentations in the presence or absence of terbinafine supplementation, respectively. These results suggest that the Y2805 strain has potential as a new alternative source of squalene production.