Heterologous production of a new lasso peptide brevunsin in Sphingomonas subterranea. (1st November 2018)

• Record Type: Journal Article
• Authors: Kodani, Shinya; Hemmi, Hikaru; Miyake, Yuto; Kaweewan, Issara; Nakagawa, Hiroyuki
• Is Part Of: Journal of industrial microbiology & biotechnology. Volume 45:Number 11(2018)
• Page Start: 983
• Page End: 992
• Publication Date: 2018-11-01
• Subjects: Lasso peptide -- Heterologous production -- Sphingomonas subterranea -- Brevundimonas diminuta -- NMR; Industrial microbiology -- Periodicals; 660.62
• DOI: 10.1007/s10295-018-2077-6
• Languages: English
• ISSNs: 1367-5435

Abstract

Abstract: A shuttle vector pHSG396Sp was constructed to perform gene expression using Sphingomonas subterranea as a host. A new lasso peptide biosynthetic gene cluster, derived from Brevundimonas diminuta, was amplified by PCR and integrated to afford a expression vector pHSG396Sp-12697L. The new lasso peptide brevunsin was successfully produced by S. subterranea, harboring the expression vector, with a high production yield (10.2 mg from 1 L culture). The chemical structure of brevunsin was established by NMR and MS/MS experiments. Based on the information obtained from the NOE experiment, the three-dimensional structure of brevunsin was determined, which indicated that brevunsin possessed a typical lasso structure. This expression vector system provides a new heterologous production method for unexplored lasso peptides that are encoded by bacterial genomes.