Bioprocess engineering to produce 9-(nonanoyloxy) nonanoic acid by a recombinant Corynebacterium glutamicum-based biocatalyst. (1st September 2017)

• Record Type: Journal Article
• Authors: Kim, Hyeonsoo; Park, Soohyun; Cho, Sukhyeong; Yang, Jeongmo; Jeong, Kijun; Park, Jinbyung; Lee, Jinwon
• Is Part Of: Journal of industrial microbiology & biotechnology. Volume 44:Number 9(2017)
• Page Start: 1301
• Page End: 1311
• Publication Date: 2017-09-01
• Subjects: 10-Ketostearic acid -- 9-(Nonanoyloxy) nonanoic acid -- Corynebacterium glutamicum -- Biocatalyst; Industrial microbiology -- Periodicals; 660.62
• DOI: 10.1007/s10295-017-1945-9
• Languages: English
• ISSNs: 1367-5435

Abstract

Abstract: Here, Corynebacterium glutamicum ATCC13032 expressing Baeyer–Villiger monooxygenase from Pseudomonas putida KT2440 was designed to produce 9-(nonanoyloxy) nonanoic acid from 10-ketostearic acid. Diverse parameters including cultivation and reaction temperatures, type of detergent, and pH were found to improve biotransformation efficiency. The optimal temperature of cultivation for the production of 9-(nonanoyloxy) nonanoic acid from 10-ketostearic acid using whole cells of recombinant C. glutamicum was 15 °C, but the reaction temperature was optimal at 30 °C. Enhanced conversion efficiency was obtained by supplying 0.05 g/L of Tween 80 at pH 7.5. Under these optimal conditions, recombinant C. glutamicum produced 0.28 mM of 9-(nonanoyloxy) nonanoic acid with a 75.6% (mol/mol) conversion yield in 2 h. This is the first report on the biotransformation of 10-ketostearic acid to 9-(nonanoyloxy) nonanoic acid with a recombinant whole-cell C. glutamicum -based biocatalyst and the results demonstrate the feasibility of using C. glutamicum as a whole-cell biocatalyst.