Engineering deacetoxycephalosporin C synthase as a catalyst for the bioconversion of penicillins. (1st May 2017)

• Record Type: Journal Article
• Authors: Fan, Keqiang; Lin, Baixue; Tao, Yong; Yang, Keqian
• Is Part Of: Journal of industrial microbiology & biotechnology. Volume 44:Number 4/5(2017)
• Page Start: 705
• Page End: 710
• Publication Date: 2017-05-01
• Subjects: Deacetoxycephalosporin C synthase (DAOCS) -- Penicillin expandase -- 7-aminodeacetoxycephalosporanic acid (7-ADCA) -- Bioconversion -- Penicillin G; Industrial microbiology -- Periodicals; 660.62
• DOI: 10.1007/s10295-016-1857-0
• Languages: English
• ISSNs: 1367-5435

Abstract

Abstract: 7-aminodeacetoxycephalosporanic acid (7-ADCA) is a key intermediate of many clinically useful semisynthetic cephalosporins that were traditionally prepared by processes involving chemical ring expansion of penicillin G. Bioconversion of penicillins to cephalosporins using deacetoxycephalosporin C synthase (DAOCS) is an alternative and environmentally friendly process for 7-ADCA production. Arnold Demain and co-workers pioneered such a process. Later, protein engineering efforts to improve the substrate specificity and catalytic efficiency of DAOCS for penicillins have been made by many groups, and a whole cell process using Escherichia coli for bioconversion of penicillins has been developed.