Engineering Yarrowia lipolytica for poly-3-hydroxybutyrate production. (1st May 2017)

• Record Type: Journal Article
• Authors: Li, Zheng-Jun; Qiao, Kangjian; Liu, Nian; Stephanopoulos, Gregory
• Is Part Of: Journal of industrial microbiology & biotechnology. Volume 44:Number 4/5(2017)
• Page Start: 605
• Page End: 612
• Publication Date: 2017-05-01
• Subjects: Poly-3-hydroxybutyrate -- PHB -- Metabolic engineering -- phaCAB -- Acetate -- Yarrowia lipolytica; Industrial microbiology -- Periodicals; 660.62
• DOI: 10.1007/s10295-016-1864-1
• Languages: English
• ISSNs: 1367-5435

Abstract

Abstract: Strains of Yarrowia lipolytica were engineered to express the poly-3-hydroxybutyrate (PHB) biosynthetic pathway. The genes for β-ketothiolase, NADPH-dependent acetoacetyl-CoA reductase, and PHB synthase were cloned and inserted into the chromosome of Y. lipolytica . In shake flasks, the engineered strain accumulated PHB to 1.50 and 3.84% of cell dry weight in complex medium supplemented with glucose and acetate as carbon source, respectively. In fed-batch fermentation using acetate as sole carbon source, 7.35 g/l PHB (10.2% of cell dry weight) was produced. Selection of Y. lipolytica as host for PHB synthesis was motivated by the fact that this organism is a good lipids producer, which suggests robust acetyl-CoA supply also the precursor of the PHB pathway. Acetic acid could be supplied by gas fermentation, anaerobic digestion, and other low-cost supply route.