Genome-wide analyses reveal footprints of divergent selection and popping-related traits in CIMMYT's maize inbred lines. (18th October 2020)

• Record Type: Journal Article
• Authors: Li, Jing; Li, Delin; Espinosa, Cristian Zavala; Pastor, Viridiana Trejo; Rasheed, Awais; Rojas, Natalia Palacios; Wang, Jiankang; Varela, Amalio Santacruz; Carolina de Almeida Silva, Natália; Schnable, Patrick S; Costich, Denise E; Li, Huihui
• Editors: Costa, Fabrizio
• Is Part Of: Journal of experimental botany. Volume 72:Number 4(2021)
• Page Start: 1307
• Page End: 1320
• Publication Date: 2020-10-18
• Subjects: EigenGWAS -- GWAS -- maize adaptation -- popping traits -- quality traits -- tropical maize landrace; Botany -- Periodicals; Botany, Experimental -- Periodicals; Plant physiology -- Periodicals; 580
• DOI: 10.1093/jxb/eraa480
• Languages: English
• ISSNs: 0022-0957

Abstract

Abstract : Sequencing-based technology reveals popping characteristics in maize, establishing a benchmark for popcorn genetics, and supporting a gradual selection process for popping traits. Abstract: Popcorn ( Zea mays L. var. Everta ) is the most ancient type of cultivated maize. However, there is little known about the genetics of popping-related traits based on genotyping-by-sequencing (GBS) technology. Here, we characterized the phenotypic variation for seven popping-related traits in maize kernels among 526 CIMMYT inbred lines (CMLs). In total, 155 083 high-quality single nucleotide polymorphism (SNP) markers were identified by a GBS approach. Several trait-associated loci were detected by genome-wide association study for color, popping expansion volume, shape, pericarp, flotation index, floury/vitreous, and protein content, explaining a majority of the observed phenotypic variance, and these were validated by a diverse panel comprising 764 tropical landrace accessions. Sixty two of the identified loci were recognized to have undergone selection. On average, there was a 55.27% frequency for alleles that promote popping in CMLs. Our work not only pinpoints previously unknown loci for popping-related traits, but also reveals that many of these loci have undergone selection. Beyond establishing a new benchmark for the genetics of popcorn, our study provides a foundation for gene discovery and breeding. It also presents evidence to investigate the role of a gradual loss of popping ability as a by-product of diversification of culinary uses throughout the evolution of teosinte–to–modern maize.