Purification and characterization of a highly viscous polysaccharide produced by Paenibacillus strain. (April 2018)

• Record Type: Journal Article
• Authors: Xu, Haiyang; Li, Jing; Wang, Lei; Fu, Renjie; Cheng, Rui; Wang, Shiming; Zhang, Jianfa
• Is Part Of: European polymer journal. Volume 101(2018)
• Page Start: 314
• Page End: 323
• Publication Date: 2018-04
• Subjects: Polysaccharide -- Structure -- Nuclear magnetic resonance -- Rheology; Polymers -- Periodicals; Polymerization -- Periodicals; Polymères -- Périodiques; Polymérisation -- Périodiques; Polymerization; Polymers; Periodicals; Electronic journals; 547.705
• DOI: 10.1016/j.eurpolymj.2018.02.040
• Languages: English
• ISSNs: 0014-3057

Abstract

Graphical abstract: Highlights: Structure of a 7.3 × 10 6 Da polysaccharide produced by Paenibacillus strain was determined. The purified polysaccharide PA showed anti-shear, thermo- and saline-tolerant abilities. PA is a potential additive for rheological modification, such as thickening and stabilizing. Abstract: The linear, 7.3 × 10 6 Da polysaccharide (PA) was purified from the extracellular polymeric product of Paenibacillus mucilaginosus WL412. Rheological results revealed that PA solution was typical shear-thinning fluid and it was more viscous than xanthan gum solution under the same conditions. Besides, PA formed a weak gel-like structure and the anti-shear, heat-resistant, and saline tolerant abilities were preferable to xanthan gum in the testing temperature range of 10–85 °C on the basis of thixotropy and oscillation analyses. Afterwards, the chemical structure of PA was elucidated by means of methylation-GC/MS, and 1D- and 2D-NMR spectra. PA consisted of three types of glycosides, namely mannoside, glucoside, and galactoside with molar ratio of 1.89:2.11:1.00. Besides, an acetyl group existed in every repeating unit. The possible structure of PA was identified as →4)α-Glc(1 → 2)α-Man(1 → 3)β-Glc(1 → 3)α-Man(6-Ac)(1 → 3)β-Gal(1→. These results indicate that PA is a potential additive for rheological modification, such as thickener and stabilizer.