Adhesion and migration of cells responding to microtopography. Issue 5 (18th August 2014)

• Record Type: Journal Article
• Authors: Estévez, Maruxa; Martínez, Elena; Yarwood, Stephen J.; Dalby, Matthew J.; Samitier, Josep
• Is Part Of: Journal of biomedical materials research. Volume 103:Issue 5(2015:May)
• Page Start: 1659
• Page End: 1668
• Publication Date: 2014-08-18
• Subjects: adhesion -- cell migration -- microstructures -- focal adhesion kinase -- cell morphology; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/jbm.a.35293
• Languages: English
• ISSNs: 1549-3296

Abstract

Abstract: It is known that cells respond strongly to microtopography. However, cellular mechanisms of response are unclear. Here, we study wild‐type fibroblasts responding to 25 µm 2 posts and compare their response to that of FAK −/− fibroblasts and fibroblasts with PMA treatment to stimulate protein kinase C (PKC) and the small g‐protein Rac. FAK knockout cells modulated adhesion number and size in a similar way to cells on topography; that is, they used more, smaller adhesions, but migration was almost completely stalled demonstrating the importance of FAK signaling in contact guidance and adhesion turnover. Little similarity, however, was observed to PKC stimulated cells and cells on the topography. Interestingly, with PKC stimulation the cell nuclei became highly deformable bringing focus on these surfaces to the study of metastasis. Surfaces that aid the study of cellular migration are important in developing understanding of mechanisms of wound healing and repair in aligned tissues such as ligament and tendon. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1659–1668, 2015.