Self‐aggregation of amphiphilic [60]fullerenyl focal point functionalized PAMAM dendrons into pseudodendrimers: DNA binding involving dendriplex formation. Issue 5 (11th August 2014)

• Record Type: Journal Article
• Authors: Hung, Cheng‐Hsiang; Chang, Wen‐Wei; Liu, Ssu‐Ching; Wu, Shang‐Jung; Chu, Chih‐Chien; Tsai, Ya‐Ju; Imae, Toyoko
• Is Part Of: Journal of biomedical materials research. Volume 103:Issue 5(2015:May)
• Page Start: 1595
• Page End: 1604
• Publication Date: 2014-08-11
• Subjects: amphiphilic dendrons -- fullerene -- gene delivery -- PAMAM dendrimer -- click chemistry; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/jbm.a.35299
• Languages: English
• ISSNs: 1549-3296

Abstract

Abstract: In this research, we successfully performed a "click" synthesis of amphiphilic poly(amido amine) dendron‐bearing fullerenyl conjugate (C60 G1 ) using a copper(I)‐catalyzed azide‐alkyne cycloaddition reaction. The strong hydrophobicity of the C60 moiety induces self‐assembly of C60 G1 into core–shell‐like "pseudodendrimers" with a uniform size distribution and positively charged peripherals. The pseudodendrimers were well‐characterized by atomic force microscopy (AFM), transmission electron microscopy, and dynamic light scattering. On the basis of electrostatic interactions, the polycationic C60 G1 assembly can serve as a nonviral gene vector. An ethidium bromide displacement assay and agarose gel electrophoresis both indicated that C60 G1 assembly forms stable complexes with the cyclic reporter gene (pEGFP‐C1) at low nitrogen‐to‐phosphorous (N/P) ratios. AFM analysis revealed a dynamic complex‐formation process, and confirmed the synthesis of C60 G1 /pEGFP‐C1 hybrids with a particle dimensions less than 200 nm. Fluorescence microscopy and flow cytometry revealed that 51% of HeLa and 43% of MCF‐7 cells are positive to the YOYO‐1‐labeled hybrids at an N/P ratio of 2, being comparable to TurboFect‐mediated delivery. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1595–1604, 2015.