Improvement of l-citrulline production in Corynebacterium glutamicum by ornithine acetyltransferase. (1st February 2015)

• Record Type: Journal Article
• Authors: Hao, N; Mu, Jr; Hu, N; Xu, S; Yan, M; Li, Y; Guo, K; Xu, L
• Is Part Of: Journal of industrial microbiology & biotechnology. Volume 42:Number 2(2015)
• Page Start: 307
• Page End: 313
• Publication Date: 2015-02-01
• Subjects: l-Citrulline -- Corynebacterium glutamicum -- Ornithine acetyltransferase -- argJ -- Feedback inhibition; Industrial microbiology -- Periodicals; 660.62
• DOI: 10.1007/s10295-014-1561-x
• Languages: English
• ISSNs: 1367-5435

Abstract

Abstract: In this study, Corynebacterium glutamicum ATCC 13032 was engineered to produce l -citrulline through a metabolic engineering strategy. To prevent the flux away from l -citrulline and to increase the expression levels of genes involved in the citrulline biosynthesis pathway, the argininosuccinate synthase gene ( argG ) and the repressor gene ( argR ) were inactivated. The engineered C. glutamicum ATCC 13032 ∆ argG ∆ argR (CIT 2) produced higher amounts of l -citrulline (5.43 g/L) compared to the wildtype strain (0.15 g/L). To determine new strategies for further enhancement of l -citrulline production, the effect of l -citrulline on ornithine acetyltransferase (EC 2.3.1.35; OATase; ArgJ) was first investigated. Citrulline was determined to inhibit Ornithine acetyltransferase; for 50 % inhibition, citrulline concentration was 30 mM. The argJ gene from C. glutamicum ATCC 13032 was cloned, and the recombinant shuttle plasmid pXMJ19- argJ was constructed and expressed in C. glutamicum ATCC 13032 ∆ argG ∆ argR (CIT 2). Overexpression of the argJ gene exhibited increased OAT activity and resulted in a positive effect on citrulline production (8.51 g/L). These results indicate that OAT plays a vital role during l -citrulline production in C. glutamicum .