Isolation and structure determination of a new lantibiotic cinnamycin B from Actinomadura atramentaria based on genome mining. (1st August 2016)

• Record Type: Journal Article
• Authors: Kodani, Shinya; Komaki, Hisayuki; Ishimura, Sho; Hemmi, Hikaru; Ohnishi-Kameyama, Mayumi
• Is Part Of: Journal of industrial microbiology & biotechnology. Volume 43:Number 8(2016)
• Page Start: 1159
• Page End: 1165
• Publication Date: 2016-08-01
• Subjects: Actinomadura atramentaria NBRC 14695 -- Lantibiotic -- NMR spectrum -- MS/MS analysis -- Genome mining; Industrial microbiology -- Periodicals; 660.62
• DOI: 10.1007/s10295-016-1788-9
• Languages: English
• ISSNs: 1367-5435

Abstract

Abstract: New lantibiotic cinnamycin B was isolated from the extract of Actinomadura atramentaria NBRC 14695 T, based on genome mining and chemical investigation. The partial structure of cinnamycin B was established by 2D NMR experiments, which indicated that cinnamycin B had same methyl lanthionine bridging pattern with cinnamycin. The reduction with NaBH4 -NiCl2 afforded the reduced cinnamycin B, and MS/MS experiment indicated the presence of hydroxy asparatic acid in the molecule. Cinnamycin B showed an antibacterial activity against Streptomyces antibioticus with dosage of 5 μg (0.5μL, 10 mg/mL solution) at spot-on-lawn testing method. The gene cluster of cinnamycin B on the genome of A. atramentaria was identified and discussed in comparison with that of cinnamycin.