Enhanced succinic acid productivity by expression of mgtCB gene in Escherichia coli mutant. (1st April 2016)

• Record Type: Journal Article
• Authors: Wang, Jing; Yang, Le; Wang, Dan; Dong, Lichun; Chen, Rachel
• Is Part Of: Journal of industrial microbiology & biotechnology. Volume 43:Number 4(2016)
• Page Start: 505
• Page End: 516
• Publication Date: 2016-04-01
• Subjects: Succinic acid -- Magnesium transporter -- MgtC -- Alkaline neutralizer -- Productivity; Industrial microbiology -- Periodicals; 660.62
• DOI: 10.1007/s10295-015-1720-8
• Languages: English
• ISSNs: 1367-5435

Abstract

Abstract: In this study, a novel engineering Escherichia coli strain (CBMG111) with the expression of mgtCB gene was constructed for the enhanced fermentative production of succinic acid by utilizing the synergetic effect of mgtC gene to improve the growth of strains at the environment of low Mg 2+ concentration and mgtB to enhance the transport of Mg 2+ into cells. After the effect of the expression of the individual genes ( mgtA, mgtB, mgtC ) on the growth of E. coli was clarified, the fermentative production of succinic acid by CBMG111 was studied with the low-price mixture of Mg(OH)2 and NH3 ·H2 O as the alkaline neutralizer and the biomass hydrolysates as the carbon sources, which demonstrated that the expression of mgtCB gene can significantly increase the productivity of succinic acid (2.97 g L −1 h −1 ) compared with that by using the engineering strain with the overexpression of mgtA gene.