Synergistic nourishing 'Yin' effect of iridoid and phenylpropanoid glycosides from Radix Scrophulariae in vivo and in vitro. (10th January 2020)

• Record Type: Journal Article
• Authors: Gong, Pu-yang; He, Yu-wei; Qi, Jin; Chai, Cheng-zhi; Yu, Bo-yang
• Is Part Of: Journal of ethnopharmacology. Volume 246(2019)
• Publication Date: 2020-01-10
• Subjects: Radix scrophulariae -- Nourishing 'yin' -- Immunoregulation -- Antioxidant -- Synergistic effect; TCM traditional Chinese medicine -- EGP Eight Guiding Principles -- RS Radix Scrophularia -- IG iridoid glycosides -- PG phenylpropanoid glycosides -- YD 'Yin' deficiency -- HPLC-Q-TOF-MS High Performance Liquid Chromatography coupled with tandem quadrupole time-of-flight mass spectrometry -- SOD superoxide dismutase -- MDA malondialdehyde -- LPO lipid peroxidation -- cAMP cyclic adenosine monophosphate -- cGMP cyclic guanosine monophosphate -- IL-6 interleukelin-6 -- INF-γ interferon gamma -- SC synergistic coefficient -- CMC-Na Sodium carboxymethylcellulose -- FBS fetal bovine serum -- PV pharmacodynamic value; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2019.112209
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Radix Scrophulariae (RS), is a renowned traditional Chinese medicine used as nourishing ' Yin '. The iridoid glycosides (IG) and phenylpropanoid glycosides (PG) are main chemical constituents in RS. However, there had been no pharmacological experiment studies of synergy between IG and PG. Due to the constituents interactions, exploring their synergy profile is of great important for explaining the essence of nourishing ' Yin ' efficacy of RS. Aim of study: The present study was undertaken to evaluate synergistic nourishing ' Yin ' effect of IG and PG from RS in vivo and in vitro through their immunoregulation and antioxidant activities. Materials and methods: In this study, IG and PG fractions in RS were isolated and identified by High Performance Liquid Chromatography coupled with tandem quadrupole time-of-flight Mass Spectrometry (HPLC-Q-TOF-MS). The synergistic nourishing ' Yin ' effect of two fractions were investigated in vivo and in vitro with thyroxine-induced ' Yin ' deficiency (YD) mice model and primary splenic lymphocyte, respectively. The exterior syndrome signs and serologic and cellular biomarkers changes were detected. Then, the synergistic coefficient (SC) of IG and PG on every pharmacodynamics index were calculated by Webb method. Results: Compared with model and mono-therapy group (IG or PG group), IG combined with PG group significantly ameliorated YD by exerting immunoregulation and antioxidant effects. Based on the SC, IG and PG possessed a synergistic effect on heart rate, average speed, upright times, spleen index, LPO, SOD, IL-6, Na + -K + -ATP enzyme in vivo, and cAMP/cGMP, IFN-γ/IL-10, and MDA in vitro with SC > 1. Conclusions: The nourishing ' Yin ' benefits were clearly produced when IG and PG were used in combination, which provided the scientific evidence of multiple-components and multiple-approach synergistic effect of Chinese traditional herbal medicine to control and management of diseases. Graphical abstract: Image 1