Detection of smell print differences between nonmalignant and malignant prostate cells with an electronic nose. (September 2012)

• Record Type: Journal Article
• Authors: Roine, Antti; Tolvanen, Mikko; Sipiläinen, Miki; Kumpulainen, Pekka; Helenius, Merja A; Lehtimäki, Terho; Vepsäläinen, Jouko; Keinänen, Tuomo A; Häkkinen, Merja R; Koskimäki, Juha; Veskimäe, Erik; Tuokko, Antti; Visakorpi, Tapio; Tammela, Teuvo L; Sioris, Thanos; Paavonen, Timo; Lekkala, Jukka; Helle, Hannu; Oksala, Niku KJ
• Is Part Of: Future oncology. Volume 8:Number 9(2012)
• Page Start: 1157
• Page End: 1165
• Publication Date: 2012-09
• Subjects: cell lines -- electronic nose -- machine olfaction -- olfactory detection -- prostate cancer -- volatile organic compounds; Oncology -- Periodicals; 616.99405
• DOI: 10.2217/fon.12.93
• Languages: English
• ISSNs: 1479-6694

Abstract

Aim: To determine whether an electronic nose can differentiate cultured nonmalignant and malignant prostatic cells from each other and whether the smell print is secreted to the surrounding medium.Materials & methods: Prostatic nonmalignant (EP-156T and controls) and malignant (LNCaP) cell lines, as well as conditioned and unconditioned media, were collected. The smell prints of the samples were analyzed by a ChemPro ® 100 electronic nose device. The data were normalized and dimension reduction was conducted. The samples were classified and misclassification rates were calculated.Results: The electronic nose differentiated the nonmalignant and malignant cell lines from each other, achieving misclassification rates of 2.9–3.6%. Cells did not differ from the conditioned medium but differed from the unconditioned medium (misclassification rates: 0.0–25.6%).Conclusion: Malignant and nonmalignant prostatic cell lines have distinct smell prints. Prostatic cancer cells seem to modify the smell print of their medium.