Human papillomavirus DNA in head and neck squamous cell carcinomas in the Free State, South Africa. Issue 2 (9th December 2019)

• Record Type: Journal Article
• Authors: Bulane, Atang; Goedhals, Dominique; Seedat, Riaz Y.; Goedhals, Jacqueline; Burt, Felicity
• Is Part Of: Journal of medical virology. Volume 92:Issue 2(2020)
• Page Start: 227
• Page End: 233
• Publication Date: 2019-12-09
• Subjects: formalin‐fixed paraffin‐embedded tissues -- head, and neck squamous cell carcinomas -- human papillomaviruses -- hypopharynx carcinoma -- larynx carcinoma -- nasopharynx carcinoma -- oral squamous cell carcinoma -- oropharynx carcinoma -- prevalence -- sinonasal carcinoma; Virology -- Periodicals; 616
• DOI: 10.1002/jmv.25556
• Languages: English
• ISSNs: 0146-6615

Abstract

Abstract: Human papillomaviruses (HPVs) have been associated with a subset of head and neck squamous cell carcinomas (HNSCCs). The aim of this study was to determine the prevalence of HPV DNA in archived formalin‐fixed paraffin‐embedded tissue from patients with histologically confirmed HNSCCs in a South African cohort. A nested PCR was used for the detection of HPV DNA targeting the L1 gene. Positive samples were confirmed using an in‐house hemi‐nested PCR targeting the E6 gene and genotyped by sequence determination of amplicons. HPV DNA was detected in 57/780 (7.3%) samples, with the highest prevalence being in the sinonasal tract (16.0%) and oropharynx (10.8%). HPV16 was the most frequently detected type, being found in 26/57 (45.6%) positive samples. The prevalence of HPV DNA in HNSCCs found in this study was lower than that found in developed countries