The contribution of MYC and PLK1 expression to proliferative capacity in diffuse large B-cell lymphoma. Issue 13 (10th November 2019)

• Record Type: Journal Article
• Authors: Oon, Ming Liang; Hoppe, Michal M.; Fan, Shuangyi; Phyu, The; Phuong, Hoang M.; Tan, Soo-Yong; Hue, Susan Swee-Shan; Wang, Shi; Poon, Li M.; Chan, Hian L. E.; Lee, Joanne; Chee, Yen L.; Chng, Wee-Joo; de Mel, Sanjay; Liu, Xin; Jeyasekharan, Anand D.; Ng, Siok-Bian
• Is Part Of: Leukemia & lymphoma. Volume 60:Issue 13(2019)
• Page Start: 3214
• Page End: 3224
• Publication Date: 2019-11-10
• Subjects: Diffuse large B cell lymphoma -- MYC -- PLK1 -- Ki67 -- proliferation -- fluorescence immunohistochemistry; Leukemia -- Periodicals; Lymphomas -- Periodicals; 616.99419
• DOI: 10.1080/10428194.2019.1633629
• Languages: English
• ISSNs: 1042-8194

Abstract

Abstract: Polo-like kinase-1 (PLK1) regulates the MYC-dependent kinome in aggressive B-cell lymphoma. However, the role of PLK1 and MYC toward proliferation in diffuse large B-cell lymphoma (DLBCL) is unknown. We use multiplexed fluorescent immunohistochemistry (fIHC) to evaluate the co-localization of MYC, PLK1 and Ki67 to study their association with proliferation in DLBCL. The majority (98%, 95% CI 95–100%) of MYC/PLK1-double positive tumor cells expressed Ki67, underscoring the key role of the MYC/PLK1 circuit in proliferation. However, only 38% (95% CI 23–40%) and 51% (95% CI 46–51%) of Ki67-positive cells expressed MYC and PLK1, respectively. Notably, 40% (95% CI 26–43%) of Ki67-positive cells are MYC- and PLK-negative. A stronger correlation exists between PLK1 and Ki67 expression ( R = 0.74, p < .001) than with MYC and Ki67 expression ( R = 0.52, p < .001). Overall, the results indicate that PLK1 has a higher association than MYC in DLBCL proliferation and there are mechanisms besides MYC and PLK1 influencing DLBCL proliferation.