Mechanism of the lifespan extension induced by submaximal SERCA inhibition in C. elegans. (June 2021)

• Record Type: Journal Article
• Authors: García-Casas, Paloma; Alvarez-Illera, Pilar; Fonteriz, Rosalba I.; Montero, Mayte; Alvarez, Javier
• Is Part Of: Mechanisms of ageing and development. Volume 196(2021)
• Publication Date: 2021-06
• Subjects: ER endoplasmic reticulum -- SERCA Sarco Endoplasmic Reticulum Ca2+ ATPase -- AMPK AMP-activated protein kinase -- TOR target of rapamycin -- TORC1 TOR complex 1 -- InsP3R inositol 14, 5-trisphosphate receptors -- RyR ryanodine receptors -- MAMs mitochondria-associated ER membranes -- STIM stromal interaction molecule -- NGM nematode growth medium -- 2, 5-BHQ 2, 5-di-tert-butylhydroquinone -- GFP green fluorescent protein; C. elegans -- SERCA -- Thapsigargin -- Lifespan -- Endoplasmic reticulum -- Mitochondria -- AMP kinase -- TOR; Aging -- Periodicals; Developmental biology -- Periodicals; Aging -- Periodicals; Developmental Biology -- Periodicals; Vieillissement -- Périodiques; Biologie du développement -- Périodiques; Aging; Developmental biology; Periodicals; 612.67
• DOI: 10.1016/j.mad.2021.111474
• Languages: English
• ISSNs: 0047-6374

Abstract

Highlights: Submaximal SERCA inhibition with inhibitors or RNAi increases C . elegans lifespan. Lifespan extension required functional mitochondria, and both AMPK and TOR pathways. Instead, the insulin signaling pathway and the sirtuin pathway were not involved. ER-mitochondria Ca 2+ signaling may play a role in the aging process. The SERCA pump may be a new pharmacological target to act on longevity. Abstract: We have reported recently that submaximal inhibition of the Sarco Endoplasmic Reticulum Ca 2+ ATPase (SERCA) produces an increase in the lifespan of C. elegans worms. We have explored here the mechanism of this increased survival by studying the effect of SERCA inhibition in several mutants of signaling pathways related to longevity. Our data show that the mechanism of the effect is unrelated with the insulin signaling pathway or the sirtuin activity, because SERCA inhibitors increased lifespan similarly in mutants of these pathways. However, the effect required functional mitochondria and both the AMP kinase and TOR pathways, as the SERCA inhibitors were ineffective in the corresponding mutants. The same effects were obtained after reducing SERCA expression with submaximal RNAi treatment. The SERCA inhibitors did not induce ER-stress at the concentrations used, and their effect was not modified by inactivation of the OP50 bacterial food. Altogether, our data suggest that the effect may be due to a reduced ER-mitochondria Ca 2+ transfer acting via AMPK activation and mTOR inhibition to promote survival.