Metabolic Activity Interferometer: A Powerful Tool for Testing Antibiotics. (13th May 2012)

• Record Type: Journal Article
• Authors: Machado, Rachel R. P.; Soares de Lima Filho, Elton; Dutra, Rafael C.; Barbosa Raposo, Nádia R.; Duarte, Rafael S.; Soares, Geraldo Luiz G.; Lesche, Bernhard; Kaplan, Maria Auxiliadora C.
• Other Names: Lo Yu-Lung Academic Editor.
• Is Part Of: Journal of sensors. Volume 2012(2012)
• Publication Date: 2012-05-13
• Subjects: Detectors -- Periodicals; 681.205
• DOI: 10.1155/2012/493797
• Languages: English
• ISSNs: 1687-725X

Abstract

Abstract : It is demonstrated that the efficiency of antibiotics can be tested using an interferometric method. Two antibiotics were used as models to show that an interferometric method to monitor the metabolic activity of slowly growing bacteria can be a safer method to judge antimicrobial properties of substances than conventional methods. The susceptibility of Mycobacterium bovis to hexane extract of Pterodon emarginatus and to the well-known antibiotic rifampicin was tested with the interferometric method and with the conventional microplate method. The microplate method revealed a potential activity of hexane extract against M. bovis . However, the interferometric method showed that the action of this substance is rather limited. Also in the case of rifampicin, the interferometric method was able to detect resistant bacteria.