Engineering of Nucleic Acids and Synthetic Cofactors as Holo Sensors for Probing Signaling Molecules in the Cellular Membrane Microenvironment. Issue 20 (20th March 2019)

• Record Type: Journal Article
• Authors: Feng, Guangfu; Luo, Xingyu; Lu, Xu; Xie, Shiyi; Deng, Lu; Kang, Wenyuan; He, Fang; Zhang, Jiaheng; Lei, Chunyang; Lin, Bin; Huang, Yan; Nie, Zhou; Yao, Shouzhuo
• Is Part Of: Angewandte Chemie international edition. Volume 58:Issue 20(2019)
• Page Start: 6590
• Page End: 6594
• Publication Date: 2019-03-20
• Subjects: bioimaging -- DNA-based sensor -- nucleic acids -- signaling molecules -- synthetic cofactor; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201901320
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: The comprehensive understanding of the mechanisms underlying the interaction of cells with their membrane microenvironment is of great value for fundamental biological research; however, tracking biomolecules on cell surfaces with high temporal and spatial resolution remains a challenge. Herein, a modular strategy is presented for the construction of cell surface DNA‐based sensors by engineering DNA motifs and synthetic cofactors. In this strategy, a stimuli‐reactive organic molecule is employed as the cofactor for the DNA motif, and the self‐assembly of them forms a FRET‐based holo DNA‐based sensor. With the use of the DNA‐based sensors, the versatility of this modular strategy has been demonstrated in the ratiometric imaging of the cellular extrusion process of endogenous signaling molecules, including sulfur dioxide derivatives and nitric oxide. Abstract : Makes sense to me : A modular strategy for the construction of cell surface DNA‐based sensors is presented, which involves engineering DNA motifs and synthetic cofactors. By using these DNA‐based sensors, ratiometric imaging of the release of endogenous signaling molecules (including sulfur dioxide derivatives and nitric oxide) into the extracellular environment is achieved.