Detection of Thyroid Carcinoma Antigen with Quantum Dots and Monoclonal IgM Antibody (JT-95) System. (27th May 2010)

• Record Type: Journal Article
• Authors: Fujioka, Kouki; Manabe, Noriyoshi; Nomura, Mayumi; Watanabe, Michiko; Takeyama, Hiroshi; Hoshino, Akiyoshi; Hanada, Sanshiro; Yamamoto, Kenji; Manome, Yoshinobu
• Other Names: Lin Chao Academic Editor.
• Is Part Of: Journal of nanomaterials. Volume 2010(2010)
• Publication Date: 2010-05-27
• Subjects: Nanostructured materials -- Periodicals; Nanotechnology -- Periodicals; Nanomatériaux; Nanostructured materials; Nanotechnology; Nanostructures; Nanotechnology; Periodicals; Fulltext; Internet Resources; Periodicals; 620.115
• DOI: 10.1155/2010/937684
• Languages: English
• ISSNs: 1687-4110

Abstract

Abstract : High-intensity fluorescent nanoparticles, quantum dots (QDs), have been applied to a wide range of biological studies and medical studies by taking advantage of their fluorescent properties. On the other hand, we have reported the specificity of JT-95 monoclonal IgM antibody, which recognizes the antigen of thyroid carcinomas. Here we show that the combination of QDs and JT-95 monoclonal antibody was applicable to Western blotting analysis, ELISA-like system, and fluorescent microscopic analysis of SW1736 thyroid carcinoma cell line. We have opened up the possibility that antibodies for higher specific recognition, even IgM, are applicable to the detection system with QDs.