Simultaneous Determination of Clidinium Bromide and Chlordiazepoxide in Combined Dosage Forms by High-Performance Liquid Chromatography. (24th February 2013)

• Record Type: Journal Article
• Authors: Ashour, Safwan; Kattan, Nuha
• Other Names: Ruiz Medina Antonio Academic Editor.
• Is Part Of: Journal of pharmaceutics. Volume 2013(2013)
• Publication Date: 2013-02-24
• Subjects: Pharmaceutical industry -- Periodicals; Drug Industry; Technology, Pharmaceutical; Pharmaceutical industry; Periodicals; 615.1
• DOI: 10.1155/2013/417682
• Languages: English
• ISSNs: 2090-9918

Abstract

Abstract : A sensitive and precise RP-HPLC method has been developed for the simultaneous estimation of clidinium bromide (CDB) and chlordiazepoxide (CDZ) in pure and pharmaceutical formulations. The separation was achieved on a Nucleodur C8 (250 × 4.6 mm i.d., 5 μ m particle size) column at 25°C. CH3 CN-MeOH-NH4 OAc 0.1M (30 : 40 : 30, v/v/v) was used as the mobile phase at a flow rate of 1.0 mL min −1 and detector wavelength at 218 nm. Almotriptan (ALT) was used as internal standard. The validation of the proposed method was carried out for linearity, accuracy, precision, LOD, LOQ, and robustness. The method showed good linearity in the ranges of 2.5–300.0 and 3.0–500.0 μ g mL −1 for CDB and CDZ, respectively. The percentage recovery obtained for CDB and CDZ was 100.40–103.38 and 99.98–105.59%, respectively. LOD and LOQ were 0.088 and 0.294 μ g mL −1 for CDB and 0.121 and 0.403 μ g mL −1 for CDZ, respectively. The proposed method was successfully applied to the determination of CDB and CDZ in combined dosage forms and the results tallied well with the label claim.