A rapid and sensitive LC–ESI-MS/MS method for the detection of YF-49–92.MLS in rat plasma. (October 2013)

• Record Type: Journal Article
• Authors: Gao, Qin; Huang, Xin; Jiang, Zhenzhou; Liu, Guanlan; Xiao, Yi; Sun, Lixin; Wang, Tao; Zhang, Luyong; Xie, Shaofei; Huang, Haiyan
• Is Part Of: Bioanalysis. Volume 5:Number 20(2013)
• Page Start: 2521
• Page End: 2530
• Publication Date: 2013-10
• Subjects: Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.13.204
• Languages: English
• ISSNs: 1757-6180

Abstract

Background: YF-49–92.MLS is a novel candidate for TB treatment. An accurate, precise and specific LC–MS/MS method for the quantification of YF-49–92.MLS in rat plasma using verapamil as an IS is reported in this paper.Methodology: Proper retention time and excellent peak shape were acquired using an Agilent Zorbax ® SB-C18 column with the mobile phase of 5 mmol/l ammonium acetate, 0.1% formic acid–methanol (30:70, v/v). The LLOQ was 1 ng/ml. The calibration curves encompassed concentrations from 20 to 5000 ng/ml. Intra- and inter-assay precision and accuracy were within 15% by determining low, medium and high concentration samples. Extraction recovery, stability, and matrix effects were also fully validated.Conclusion: This method has been validated to be rapid and sensitive, and successfully applied to the PK study of YF-49-92.MLS in rat plasma.