Impact of organic solvent additive on the integrity of plasma samples in bioanalysis by LC–MS/MS. (October 2013)
- Record Type:
- Journal Article
- Title:
- Impact of organic solvent additive on the integrity of plasma samples in bioanalysis by LC–MS/MS. (October 2013)
- Main Title:
- Impact of organic solvent additive on the integrity of plasma samples in bioanalysis by LC–MS/MS
- Authors:
- Dicaire, Catherine
Mess, Jean-Nicholas
Furtado, Milton
Bergeron, Annik
Garofolo, Fabio - Abstract:
- Background: Matrix effects are one of the major drawbacks of ESI–MS/MS. It is majorly caused by lipids in plasma, which can be overcome by using different extraction techniques.Results: In this investigation, a major matrix effect was observed in samples containing a co-administered drug. Unknown compounds appeared over time in the human plasma samples spiked with co-administered drug creating major ion suppression. The changes in matrix integrity were associated with the organic solvent content in the plasma samples.Conclusion: The amount and type of organic solvent added to human plasma along with the storage conditions must be carefully determined during method development in order not to impact quantitation.
- Is Part Of:
- Bioanalysis. Volume 5:Number 19(2013)
- Journal:
- Bioanalysis
- Issue:
- Volume 5:Number 19(2013)
- Issue Display:
- Volume 5, Issue 19 (2013)
- Year:
- 2013
- Volume:
- 5
- Issue:
- 19
- Issue Sort Value:
- 2013-0005-0019-0000
- Page Start:
- 2359
- Page End:
- 2370
- Publication Date:
- 2013-10
- Subjects:
- Drugs -- Analysis -- Periodicals
615.19005 - Journal URLs:
- http://www.future-science.com/loi/bio ↗
http://www.future-science.com/ ↗ - DOI:
- 10.4155/bio.13.207 ↗
- Languages:
- English
- ISSNs:
- 1757-6180
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - BLDSS-3PM
British Library HMNTS - ELD Digital store - Ingest File:
- 17097.xml