HILIC UHPLC–MS/MS for fast and sensitive bioanalysis: accounting for matrix effects in method development. (October 2013)

• Record Type: Journal Article
• Authors: Havlíková, Lucie; Vlčková, Hana; Solich, Petr; Nováková, Lucie
• Is Part Of: Bioanalysis. Volume 5:Number 19(2013)
• Page Start: 2345
• Page End: 2357
• Publication Date: 2013-10
• Subjects: Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.13.217
• Languages: English
• ISSNs: 1757-6180

Abstract

Background: Matrix effects are considered to be a main obstacle of quantitative bioanalytical LC–MS/MS methods. Therefore it is often required to minimize them in order to increase method reliability. HILIC has been referenced as one of possible approaches. However, there is a lack of experimental evidence in scientific literature so far.Methodology: Matrix effects were evaluated using spiked serum samples after SPE and protein precipitation prior to UHPLC–ESI-MS/MS. Chromatography was performed in both HILIC and reversed-phase mode. The influence of the matrix effects on the signal response was assessed using a set of 34 compounds of pharmaceutical interest and post-extraction addition approach.Results: The advantages and drawbacks of the HILIC and reversed-phase chromatographic modes were compared and discussed in detail.Conclusion: HILIC demonstrated the potential to reduce the occurrence of matrix effects when a more thorough sample pretreatment procedure such as SPE was applied.