Quantification of the anti-influenza drug zanamivir in plasma using high-throughput HILIC–MS/MS. (January 2011)

• Record Type: Journal Article
• Authors: Lindegardh, Niklas; Hanpithakpong, Warunee; Kamanikom, Benjamas; Farrar, Jeremy; Hien, Tran Tinh; Singhasivanon, Pratap; White, Nicholas J; Day, Nicholas P J
• Is Part Of: Bioanalysis. Volume 3:Number 2(2011)
• Page Start: 157
• Page End: 165
• Publication Date: 2011-01
• Subjects: Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.10.189
• Languages: English
• ISSNs: 1757-6180

Abstract

Background: Parenteral zanamivir is a promising drug for the treatment of severe influenza. However, quantification of this polar drug in biological matrices has traditionally been difficult and the methods developed have been relatively insensitive.Results: A high-throughput bioanalytical method for the analysis of zanamivir in human plasma using SPE in the 96-well plate format and LC coupled to positive MS/MS has been developed and validated according to US FDA guidelines. The method uses 50 µl of plasma and covers a large working range from 1–50, 000 ng/ml with a LOD of 0.50 ng/ml.Conclusion: This new LC–MS/MS assay is more sensitive than previous methods despite using a small plasma volume sample. It is particularly suitable for clinical studies on both parenteral and inhaled zanamivir.