Hydrogen Peroxide Is Involved in Salicylic Acid-Elicited Rosmarinic Acid Production in Salvia miltiorrhiza Cell Cultures. (29th May 2014)

• Record Type: Journal Article
• Authors: Hao, Wenfang; Guo, Hongbo; Zhang, Jingyi; Hu, Gege; Yao, Yaqin; Dong, Juane
• Other Names: Shao Hongbo Academic Editor.
• Is Part Of: TheScientificWorldjournal. Volume 2014(2014)
• Publication Date: 2014-05-29
• Subjects: Science -- Periodicals; Technology -- Periodicals; Medicine -- Periodicals; 505
• DOI: 10.1155/2014/843764
• Languages: English
• ISSNs: 2356-6140

Abstract

Abstract : Salicylic acid (SA) is an elicitor to induce the biosynthesis of secondary metabolites in plant cells. Hydrogen peroxide (H2 O2 ) plays an important role as a key signaling molecule in response to various stimuli and is involved in the accumulation of secondary metabolites. However, the relationship between them is unclear and their synergetic functions on accumulation of secondary metabolites are unknown. In this paper, the roles of SA and H2 O2 in rosmarinic acid (RA) production in Salvia miltiorrhiza cell cultures were investigated. The results showed that SA significantly enhanced H2 O2 production, phenylalanine ammonia-lyase (PAL) activity, and RA accumulation. Exogenous H2 O2 could also promote PAL activity and enhance RA production. If H2 O2 production was inhibited by NADPH oxidase inhibitor (IMD) or scavenged by quencher (DMTU), RA accumulation would be blocked. These results indicated that H2 O2 is secondary messenger for signal transduction, which can be induced by SA, significantly and promotes RA accumulation.