IL-7 Induces an Epitope Masking of γc Protein in IL-7 Receptor Signaling Complex. (3rd January 2017)

• Record Type: Journal Article
• Authors: Goh, Tae Sik; Jo, Yuna; Lee, Byunghyuk; Kim, Geona; Hwang, Hyunju; Ko, Eunhee; Kang, Seung Wan; Oh, Sae-Ock; Baek, Sun-Yong; Yoon, Sik; Lee, Jung Sub; Hong, Changwan
• Other Names: Cooper Dianne Academic Editor.
• Is Part Of: Mediators of inflammation. Volume 2017(2017)
• Publication Date: 2017-01-03
• Subjects: Inflammation -- Mediators -- Periodicals; Biological response modifiers -- Periodicals; Inflammation (Pathologie) -- Médiateurs; Immunomodulateurs; Biological response modifiers; Inflammation -- Mediators; Immunology; Autacoids; Immunologic Factors; Cell Adhesion Molecules; Cell Communication; Cytokines; Inflammation; Periodicals; Electronic journals; 616.0473
• DOI: 10.1155/2017/9096829
• Languages: English
• ISSNs: 0962-9351

Abstract

Abstract : IL-7 signaling via IL-7R α and common γ -chain ( γ c) is necessary for the development and homeostasis of T cells. Although the delicate mechanism in which IL-7R α downregulation allows the homeostasis of T cell with limited IL-7 has been well known, the exact mechanism behind the interaction between IL-7R α and γ c in the absence or presence of IL-7 remains unclear. Additionally, we are still uncertain as to how only IL-7R α is separately downregulated by the binding of IL-7 from the IL-7R α / γ c complex. We demonstrate here that 4G3, TUGm2, and 3E12 epitope masking of γ c protein are induced in the presence of IL-7, indicating that the epitope alteration is induced by IL-7 binding to the preassembled receptor core. Moreover, the epitope masking of γ c protein is inversely correlated with the expression of IL-7R α upon IL-7 binding, implying that the structural alteration of γ c might be involved in the regulation of IL-7R α expression. The conformational change in γ c upon IL-7 binding may contribute not only to forming the functional IL-7 signaling complex but also to optimally regulating the expression of IL-7R α .