Human Serum Albumin Cys34 Oxidative Modifications following Infiltration in the Carotid Atherosclerotic Plaque. (6th March 2014)

• Record Type: Journal Article
• Authors: Lepedda, Antonio Junior; Zinellu, Angelo; Nieddu, Gabriele; De Muro, Pierina; Carru, Ciriaco; Spirito, Rita; Guarino, Anna; Piredda, Franco; Formato, Marilena
• Other Names: Pantos Constantinos Academic Editor.
• Is Part Of: Oxidative medicine and cellular longevity. Volume 2014(2014)
• Publication Date: 2014-03-06
• Subjects: Oxidative stress -- Periodicals; Cells -- Aging -- Periodicals; Cells -- Aging; Oxidative stress; Oxidative Stress -- Periodicals; Cell Aging -- Periodicals; Periodicals; 611.0181
• DOI: 10.1155/2014/690953
• Languages: English
• ISSNs: 1942-0900

Abstract

Abstract : Objectives. To evaluate if the prooxidant environment present in atherosclerotic plaque may oxidatively modify filtered albumin. Methods. Fluorescein-5-maleimide labelled plasma samples and plaque extracts from 27 patients who had undergone carotid endarterectomy were analysed through nonreducing SDS-PAGE for albumin-Cys 34 oxidation. Furthermore, degree and pattern of S-thiolation in both circulating and plaque-filtered albumin were assayed. Results. Albumin filtered in the atherosclerotic plaque showed higher levels of Cys 34 oxidative modifications than the corresponding circulating form as well as different patterns of S-thiolation. Conclusions. Data indicate that the circulating albumin, once filtered in plaque, undergoes Cys 34 oxidative modifications and demonstrate for the first time that albumin is a homocysteine and cysteinylglycine vehicle inside the plaque environment.