Heavy oils, principally long-chain n-alkanes secreted by Aureobasidium pullulans var. melanogenum strain P5 isolated from mangrove system. (1st September 2014)

• Record Type: Journal Article
• Authors: Liu, Yuan-Yuan; Chi, Zhe; Wang, Zhi-Peng; Liu, Guang-Lei; Chi, Zhen-Ming
• Is Part Of: Journal of industrial microbiology & biotechnology. Volume 41:Number 9(2014)
• Page Start: 1329
• Page End: 1337
• Publication Date: 2014-09-01
• Subjects: Heavy oils -- Aureobasidium pullulans var. melanogenum -- Long-chain n-alkanes -- Secretion -- Mangrove system; Industrial microbiology -- Periodicals; 660.62
• DOI: 10.1007/s10295-014-1484-6
• Languages: English
• ISSNs: 1367-5435

Abstract

Abstract: In this study, the yeast strain P5 isolated from a mangrove system was identified to be a strain of Aureobasidium pullulans var. melanogenum and was found to be able to secrete a large amount of heavy oil into medium. After optimization of the medium for heavy oil production and cell growth by the yeast strain P5, it was found that 120.0 g/l of glucose and 0.1 % corn steep liquor were the most suitable for heavy oil production. During 10-l fermentation, the yeast strain P5 produced 32.5 g/l of heavy oil and cell mass was 23.0 g/l within 168 h. The secreted heavy oils contained 66.15 % of the long-chain n -alkanes and 26.4 % of the fatty acids, whereas the compositions of the fatty acids in the yeast cells were only C16:0 (21.2 %), C16:1 (2.8 %), C18:0 (2.9 %), C18:1 (39.8 %), and C18:2 (33.3 %). We think that the secreted heavy oils may be used as a new source of petroleum in marine environments. This is the first report of yeast cells which can secrete the long-chain n -alkanes.