Sildenafil and tadalafil reduce the risk of contrast-induced nephropathy by modulating the oxidant/antioxidant balance in a murine model. (January 2020)

• Record Type: Journal Article
• Authors: Iordache, Andrei Mihai; Docea, Anca Oana; Buga, Ana Maria; Zlatian, Ovidiu; Ciurea, Marius Eugen; Rogoveanu, Otilia Constantina; Burada, Florin; Sosoi, Simona; Mitrut, Radu; Mamoulakis, Charalampos; Albulescu, Dana; Vasile, Ramona Constantina; Tsatsakis, Aristides; Calina, Daniela
• Is Part Of: Food and chemical toxicology. Volume 135(2020)
• Publication Date: 2020-01
• Subjects: Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2019.111038
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: The aim of the study was to evaluate the potential protective role of sildenafil and tadalafil in contrast-induced nephropathy (CIN) by modulating oxidative stress. Thirty Wistar male rats were equally assigned into five groups: sham, CIN, CIN + sildenafil (10 mg/kg bw/day), CIN + tadalafil (5 mg/kg bw/day) and CIN + N-Acetyl Cysteine (NAC) (100 mg/kg bw/day) as a positive control. CIN was induced by 12 h dehydration and administration of indomethacin (10 mg/kg bw), N-ω- nitro-L-arginine methyl ester (10 mg/kg bw), and iopromide (3 g/kg bw iodine). Blood was drawn prior to and 24 h after CIN induction for evaluating renal function and oxidative stress. In the CIN group, total antioxidant capacity (TAC), reduced glutathione (GSH) and catalase (CAT) levels were significantly decreased; and protein carbonyl (PROTC) and thiobarbituric reactive species (TBARS) were significantly increased compared to the sham group. Pre- Sildenafil and tadalafil pre-treatment reduced CIN risk and reversed oxidative stress almost to the sham group levels. These results suggest that PDE5Is can be good candidates for preventing CIN based on their ability to modulate the oxidant/antioxidant balance. Graphical abstract: Image 1 Highlights: Contrast-induced nephropathy is determined by disturbances in oxidant/antioxidant balance. TAC, GSH and CAT levels decrease while TABRS and PROTC levels increase in CIN. Sildenafil and tadalafil reduce CIN risk. Sildenafil and tadalafil increase TAC, GSH and CAT levels. Sildenafil and tadalafil decrease TBARS and PROTC levels.