Target gene expression studies on Platynereis dumerilii and Platynereis cfr massiliensis at the shallow CO2 vents off Ischia, Italy. (31st July 2018)

• Record Type: Journal Article
• Authors: Wäge, Janine; Rotchell, Jeanette M.; Gambi, Maria-Cristina; Hardege, Jörg D.
• Is Part Of: Estuarine, coastal and shelf science. Volume 207(2018)
• Page Start: 351
• Page End: 358
• Publication Date: 2018-07-31
• Subjects: Ocean acidification -- Gene expression -- CO2 vents -- Polychaeta -- P. dumerilii -- Sibling species; Estuarine oceanography -- Periodicals; Coasts -- Periodicals; Estuarine biology -- Periodicals; Seashore biology -- Periodicals; Coasts; Estuarine biology; Estuarine oceanography; Seashore biology; Periodicals; 551.461805
• DOI: 10.1016/j.ecss.2017.11.012
• Languages: English
• ISSNs: 0272-7714

Abstract

Abstract: Many studies predict negative effects of ocean acidification on marine organisms, potentially leading to loss of biodiversity and ecosystem function. Research on species inhabiting naturally high p CO2 environments, such as volcanic CO2 vents, offers an opportunity to understand the molecular mechanisms involved in high p CO2 regulation. Here we investigate the relative expression of NADH dehydrogenase, sodium-hydrogen antiporter (NHE), carbonic anhydrase (CA) and paramyosin genes from two non-calcifying sibling Nereididae polychaetes species, Platynereis cfr massiliensis, collected in the shallow CO2 vents off Ischia (Italy; 40°43′52.0″N 13°57′46.2″E and 40°43′55.5″N 13°57′48.4″E), and P. dumerilii collected in an area nearby (40°43′34.51″N; 13°57′35.7″E). The origin of the worms was confirmed using restriction enzyme digest. NHE and paramyosin expressions were both significantly increased in P. dumerilii relative to the P. cfr massiliensis vent populations. Furthermore, a seven day laboratory transfer experiment to lower/higher p CO2 conditions was conducted to investigate the effects on the short term gene expression. The transfer experiment of the non-vent worms to high p CO2 conditions showed no significant effect on any of the genes analysed, however, two genes ( NADH dehydrogenase and NHE ) from worms of the vent population were significantly down-regulated under low p CO2 . These findings will help to gain further insights into the cellular mechanisms affected by p CO2 changes in two polychaete species.