Large-Scale Plantlet Conversion and Ex Vitro Transplantation Efficiency of Siberian Ginseng by Bioreactor Culture. (31st October 2013)

• Record Type: Journal Article
• Authors: Yang, Jingli; Zhao, Shicheng; Yu, Changyeon; Li, Chenghao
• Other Names: Del Gaudio S. Academic Editor.; Fickers P. Academic Editor.; Govindwar S. P. Academic Editor.; Menoret S. Academic Editor.; Rodtong S. Academic Editor.
• Is Part Of: TheScientificWorldjournal. Volume 2013(2013)
• Publication Date: 2013-10-31
• Subjects: Science -- Periodicals; Technology -- Periodicals; Medicine -- Periodicals; 505
• DOI: 10.1155/2013/829067
• Languages: English
• ISSNs: 2356-6140

Abstract

Abstract : To achieve large-scale low-cost ex vitro acclimatization of Siberian ginseng plants, heart- and torpedo-shaped secondary somatic embryos (SEs) induced from germinated SEs on agar medium were collected and then inoculated to 10-l bubble column bioreactor, respectively. For plantlet conversion, inoculation of torpedo-shaped secondary SEs was more effective than heart-shaped SEs. TS2 (culture of torpedo-shaped SEs in a bioreactor with a 2-week subculture interval) plantlets had a higher root number and leaf number and larger leaf area than did HS3 (culture of heart-shaped SEs in a bioreactor with a 3-week subculture interval) and HS2 (culture of heart-shaped SEs in a bioreactor with a 2-week subculture interval) plantlets. Of these converted plants, TS2 plantlets had higher survival rate (83.7%) and growth characteristics after transplantation in a simple shed covered with a 50% sunshade net only for 6 months. TS2 plantlets also showed significantly lower H2 O2 content and significantly increased superoxide dismutase ( SOD ), glutathione peroxidase ( GPX ), and glutathione transferase ( GST ) expression levels as compared to HS2 plants when exposure to ex vitro conditions.