Efficient biodegradation of aliphatic polyester by genetically engineered strains of the yeast Yarrowia lipolytica. (July 2021)

• Record Type: Journal Article
• Authors: Kosiorowska, Katarzyna E.; Połomska, Xymena; Wang, Guokun; Borodina, Irina; Mirończuk, Aleksandra M.
• Is Part Of: International biodeterioration & biodegradation. Volume 161(2021)
• Publication Date: 2021-07
• Subjects: Cutinase -- Lipase -- Poly-ε-caprolactone -- PCL -- Yarrowia lipolytica -- Biodegradation; Biodegradation -- Periodicals; Bioremediation -- Periodicals; Biodegradation -- Periodicals; Biodégradation -- Périodiques; Biorestauration -- Périodiques; Electronic journals; 620.11223
• DOI: 10.1016/j.ibiod.2021.105232
• Languages: English
• ISSNs: 0964-8305

Abstract

Abstract: The increased production and consumption of plastic materials in today's world has caused a huge number of environmental problems, the solution of which is now crucial to improving the state of nature of our planet. In this study, we expressed cutinases from Fusarium solani f. sp. pisi and from Trichoderma reesei, individually or in combination with overexpression of the native lipase Lip2 in the yeast Yarrowia lipolytica . The engineered strains showed biodegradation activity toward polyester in the pH range between 4.0 and 9.0. The highest esterase activity was observed at pH 9.0 for the strain overexpressing cutinase from F. solani and lipase from Y. lipolytica, it reached 63.5 U ml −1, moreover this strain was capable to degrade 0.5 g of polycaprolactone film within 144 h of shake flask culture. The obtained results showed that natural capability of Y. lipolytica combined with metabolic engineering results in a highly efficient biodegradation process. Highlights: Engineered Y. lipolytica is capable of efficient and rapid polyester degradation. Almost 100% of PCL is degradated within 144 h of cultivation. Co-expression of cutinase and lipase increase the polyester degradation. Engineered Y. lipolytica utilizes PCL independently from pH of the environment.