Intestine‐on‐chip device increases ECM remodeling inducing faster epithelial cell differentiation. Issue 2 (27th November 2019)

• Record Type: Journal Article
• Authors: De Gregorio, Vincenza; Corrado, Brunella; Sbrescia, Simone; Sibilio, Sara; Urciuolo, Francesco; Netti, Paolo A.; Imparato, Giorgia
• Is Part Of: Biotechnology and bioengineering. Volume 117:Issue 2(2020)
• Page Start: 556
• Page End: 566
• Publication Date: 2019-11-27
• Subjects: 3D human intestine equivalent (3D‐HIE) -- bottom‐up tissue engineering -- extracellular matrix (ECM) -- intestine‐on‐chip; Biotechnology -- Periodicals; Bioengineering -- Periodicals; 660.6
• DOI: 10.1002/bit.27186
• Languages: English
• ISSNs: 0006-3592

Abstract

Abstract: An intestine‐on‐chip has been developed to study intestinal physiology and pathophysiology as well as intestinal transport absorption and toxicity studies in a controlled and human similar environment. Here, we report that dynamic culture of an intestine‐on‐chip enhances extracellular matrix (ECM) remodeling of the stroma, basement membrane production and speeds up epithelial differentiation. We developed a three‐dimensional human intestinal stromal equivalent composed of human intestinal subepithelial myofibroblasts embedded in their own ECM. Then, we cultured human colon carcinoma‐derived cells in both static and dynamic conditions in the opportunely designed microfluidic system until the formation of a well‐oriented epithelium. This low cost and handy microfluidic device allows to qualitatively and quantitatively detect epithelial polarization and mucus production as well as monitor barrier function and ECM remodeling after nutraceutical treatment. Abstract : By combining a tissue engineering strategy to build up functional organotypic models in vitro, with tissue on chip technology, it is proposed a low‐cost and handy Intestine‐on‐a‐chip that enhances ECM remodeling and speeds up epithelial differentiation leading to an intestinal model with more physiological TEER and mucus production. This is an effective alternative to animal model for drug as well as nutraceutical compounds testing.