Replication fork pausing at protein barriers on chromosomes. Issue 13 (30th June 2019)

• Record Type: Journal Article
• Authors: Hizume, Kohji; Araki, Hiroyuki
• Is Part Of: FEBS letters. Volume 593:Issue 13(2019)
• Page Start: 1449
• Page End: 1458
• Publication Date: 2019-06-30
• Subjects: CMG complex -- DNA polymerase -- Fob1 -- fork pausing -- fork protection complex -- helicase -- Polε -- programmed fork arrest -- replication fork -- replication fork barrier; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1002/1873-3468.13481
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : When a cell divides prior to completion of DNA replication, serious DNA damage may occur. Thus, in addition to accuracy, the processivity of the replication forks is important. DNA synthesis at replication forks should be completed in time, and forks overcome aberrant structures on the template DNA, including damaged sites, using trans‐lesion synthesis, occasionally introducing mutations. By contrast, the protein barrier built on the DNA is known to block the progression of replication forks at specific chromosomal loci. Such protein barriers avert any collision of replication and transcription machineries, or control the recombination of specific loci. The components and the mechanisms of action of protein barriers have been revealed mainly using genetic and biochemical techniques. In addition to proteins involved in replication fork pausing, the interaction of the replicative helicase and DNA polymerase is also essential for replication fork pausing. Here, we provide an overview of replication fork pausing at protein barriers. Abstract :