Intra-amniotic administration (Gallus gallus) of TiO2, SiO2, and ZnO nanoparticles affect brush border membrane functionality and alters gut microflora populations. (January 2020)

• Record Type: Journal Article
• Authors: Kolba, Nikolai; Guo, Zhongyuan; Olivas, Fabiola Moreno; Mahler, Gretchen J.; Tako, Elad
• Is Part Of: Food and chemical toxicology. Volume 135(2020)
• Publication Date: 2020-01
• Subjects: Ingestion -- Nanotoxicity -- Microbiota -- Iron -- Zinc -- Cecum; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2019.110896
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Metal oxide nanoparticles (NP) are increasingly used in the food and agriculture industries, making human consumption nearly unavoidable. The goal of this study was to use the Gallus gallus (broiler chicken) intra-amniotic administration of physiologically relevant concentrations of TiO2, SiO2, and ZnO to better understand the effects of NP exposure on gut health and function. Immediately after hatch, blood, cecum, and small intestine were collected for assessment of iron (Fe)-metabolism, zinc (Zn)-metabolism, brush border membrane (BBM) functional, and pro-inflammatory related proteins gene expression; blood Fe and Zn levels; cecum weight; and the relative abundance of intestinal microflora. NP type, dose, and the presence or absence of minerals was shown to result in altered mineral transporter, BBM functional, and pro-inflammatory gene expression. Metal oxide NP also altered the abundance of intestinal bacterial populations. Overall, the data suggest that the in vivo results align with in vitro studies, and that NP have the potential to negatively affect intestinal functionality and health. Graphical abstract: Image 1 Highlights: NP type/dose, and presence/absence of minerals altered mineral transporter, BBM functional/pro-inflammatory gene expression. Metal oxide NP altered the abundance of intestinal bacterial populations. TiO2 and SiO2 NP have the potential to negatively affect intestinal function and health.