Interaction between eNOS gene polymorphism and current smoking on susceptibility to coronary heart disease in Chinese people. Issue 1 (January 2020)

• Record Type: Journal Article
• Authors: Yang, Ping; Wu, Peng; Liu, Xing; Feng, Jian; Zheng, Shuzhan; Wang, Yan; Fan, Zhongcai
• Is Part Of: Coronary artery disease. Volume 31:Issue 1(2020:Jan.)
• Publication Date: 2020-01
• Subjects: coronary heart disease -- endothelial nitric oxide synthase -- interaction -- single-nucleotide polymorphisms -- smoking; Coronary heart disease -- Periodicals; Coronary Disease -- Indexes; Coronary Disease -- Periodicals; 616.123005
• DOI: 10.1097/MCA.0000000000000780
• Languages: English
• ISSNs: 0954-6928

Abstract

Abstract : Objective: This study aims to explore the relation between endothelial nitric oxide synthase (eNOS) single-nucleotide polymorphisms (SNPs) and the risk of coronary heart disease (CHD). Methods: SNPstats (online software: http://bioinfo.iconcologia.net/SNPstats ) was performed to test Hardy–Weinberg equilibrium in controls. Generalized multifactor dimensionality reduction (GMDR) was adopted to screen the preferable interaction between eNOS SNPs and smoking. Results: The frequency for the rs1799983-T allele was 31.1% in CHD patients, which was significantly higher than that of 19.8% in controls ( P < 0.05). The frequency for the rs891512-A allele was 28.8% in cases, which was also significantly higher than that of 20.1% in controls ( P < 0.05). Logistic regression analysis showed that both rs1799983-T and rs891512-A alleles were related with increased risk of CHD, and the odds ratios (ORs) [95% confidence interval (CI)] were 1.71 (1.31–2.15) and 1.57 (1.14–2.07), respectively. High-order interactions were investigated among SNPs and environmental factors using the GMDR method. The data showed that a two-locus model (rs1799983 × smoking) had a testing accuracy of 0.60 ( P = 0.001). We found that current smokers with rs1799983-GT or TT within eNOS gene have the highest CHD risk, compared to never smokers with rs1799983-GG genotype, OR (95% CI) = 2.74 (1.78–3.85), after covariates adjustment for age, gender, BMI, and alcohol drinking. Conclusion: The rs1799983-T and rs891512-A alleles and interaction between rs1799983 and smoking were all risk factors of CHD.