Modulatory role of exopolysaccharides of Kluyveromyces marxianus and Pichia kudriavzevii as probiotic yeasts from dairy products in human colon cancer cells. (January 2020)

• Record Type: Journal Article
• Authors: Rahbar Saadat, Yalda; Yari Khosroushahi, Ahmad; Movassaghpour, Ali Akbar; Talebi, Mehdi; Pourghassem Gargari, Bahram
• Is Part Of: Journal of functional foods. Volume 64(2020)
• Publication Date: 2020-01
• Subjects: Probiotic yeasts -- Colon cancer -- Apoptosis -- Ferroptosis -- Signaling pathways -- Exopolysaccharides; AKT Protein Kinase B -- BAX BCL2 Associated X, Apoptosis Regulator -- Bcl-2 B-cell lymphoma 2 -- cDNA complementary deoxyribonucleic acid -- CoQ10 coenzyme Q10 -- CRC colorectal cancer -- DAPI 4′, 6-diamidino-2-phenylindole -- DCF-DA 2′, 7′‐dichlorofluorescin diacetate -- DMSO dimethyl sulfoxide -- DNA deoxyribonucleic acid -- EDTA ethylenediaminetetraacetic acid -- EPS exopolysaccharide -- FAO Food and Agriculture Organization -- FBS fetal bovine serum -- FITC fluorescein isothiocyanate -- 5-FU fluorouracil -- GAPDH glyceraldehyde 3-phosphate dehydrogenase -- GIT gastrointestinal tract -- GRAS generally recognized as safe -- JAK/STAT Janus kinases/Signal Transducer and Activator of Transcription proteins -- LAB lactic acid bacteria -- MAPK mitogen-activated protein kinase -- mRNA messenger ribonucleic acid -- mTOR mammalian target of rapamycin -- NF-kB nuclear factor kappa-light-chain-enhancer of activated B cells -- Nrf-2 nuclear factor erythroid 2-related factor 2 -- PBS phosphate-buffered saline -- PCD programmed cell death -- Pen/Strep penicillin/streptomycin -- PI Propidium Iodide -- PI3K phosphoinositide 3-kinase -- PS phosphatidylserine -- q-PCR quantitative polymerase chain reaction -- QPS qualified presumption of safety -- RCD regulated cell death -- RNA ribonucleic acid -- ROS reactive oxygen species -- RPMI1640 Roswell Park Memorial Institute 1640 -- SCFA short chain fatty acid -- TCA trichloroacetic acid -- TLR toll like receptor -- WHO World Health Organization -- YMB yeast mold broth; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2019.103675
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Employing new molecular based targeted therapies by non- saccharomyces probiotic strains in CRC. Targeting important molecular pathways via EPSs of K. marxianus and P. kudriavzevii . Targeting non-apoptotic forms of cell death besides the apoptotic cell death. Abstract: Colorectal cancer (CRC) is a widespread and lethal cancer throughout the world. Nowadays there is considerable debate about the efficacy of conventional methods in CRC treatment. Though targeting vital molecular pathways, as well as induction of various forms of cell death by safe probiotic yeast components are regarded as potential therapeutic modalities against CRC. Hence, here we aimed to investigate the inhibitory role of the EPSs of Kluyveromyces marxianus and Pichia kudriavzevii on different colon cancer cell lines. In this regard, several cellular and molecular experiments were performed. Here, we found that both EPSs can induce apoptosis which were confirmed by DAPI and Annexin V/PI assays. Further, our data showed that the EPSs could not act via ferroptotic pathways, however, they could hinder the AKT-1, mTOR, and JAK-1 pathways, and induce apoptosis. Therefore, the probiotic yeast EPSs present beneficial effects and may provide as a novel molecular-targeted therapeutics for combating CRC.